– Getting Started & Next Steps
Effective DNA Sequencing for Microbial Identification
The study of microbial biology has been a focal discipline in the world for some time now. Its importance cannot be overemphasized due to the fact that understanding microbials is key to solving many human problems. Among the harmful microorganisms are bacteria, yeasts and molds. They are also not easy to identify hence they require special analysis to find them. Innovations have been brought forward to make the process of microbial identification easy.
The best tool used by microbiologists to identify microbials is DNA sequencing. DNA sequencing entails careful study and analysis of genes in a bid to narrow down and find the specific microbials. Microbial identification the world over has been made easier by the use of special DNA sequencing software that is used by most microbiologists. This is because computers have made it easier to pinpoint the genes in a crowded space of organisms. The accuracy of the results produced using software is also high and reliable.
Many laboratories in the world do microbial identification in different ways. Case in point is the use of particular software by MIDI Labs to do gene sequencing. The process that has been put in place by such laboratories is easy to follow. The first thing is the acceptance of carefully-packaged samples from customers. Secondly, the extraction process of genomic DNA from the organism will be done.
Extraction of the genomic DNA is followed by the arrangement of the strands in a double-stranded manner. Fluorescent sequencing chemistry is used in this process. The use of the latest modern chemistry ensures that this process is done well and to perfection. It is at this point that the compiled raw sequences data files are organized into consensus sequences. Software is then used for the data analysis part. A common software for most labs is Sherlock DNA Software.
From consensus sequencing, identification reports are generated. The best software used at this stage is one that has multifunctionality. This means that it should handle more than just the analysis of the genotypes in question. Sherlock DNA Software for instance can have an expanded library as well as provide for taxonomy. Using the software, it is easy to identify the microbials and actually do it expeditiously.
To get great results when it comes to microbial identification, get assistance from the best labs around. Ensure that the lab you identify is one that has experienced microbiologists and analysts. The best results will then be delivered courtesy of the experience. Having a good software that is well recognized in the world is also an added advantage. With such software, you will feel very confident with the results. Finally, ensure that they can deliver the work expeditiously. Most labs can deliver in a matter of days.